› 3l77

X-ray diffraction
1.55Å resolution

X-ray structure alcohol dehydrogenase from archaeon Thermococcus sibiricus complexed with 5-hydroxy-NADP

Released:
DOI: 10.2210/pdb3l77/pdb
PDB entry 3l77 coloured by chain, front view.
PDB entry 3l77 coloured by chain, side view.
PDB entry 3l77 coloured by chain, top view.
Source organism: Thermococcus sibiricus MM 739
Entry authors: Lyashenko AV, Lashkov AA, Gabdoulkhakov AG, Mikhailov AM

Function and Biology Details

Reaction catalysed: 
An alcohol + NADP(+) = an aldehyde + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111548 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Short-chain alcohol dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 235 amino acids
Theoretical weight: 26.32 KDa
Source organism: Thermococcus sibiricus MM 739
Expression system: Escherichia coli
UniProt:
  • Canonical: C6A190 (Residues: 1-234; Coverage: 100%)
Gene name: TSIB_0319
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

4 bound ligands:
Ligand NJP 1 x NJP
Ligand GOL 5 x GOL
Ligand PG4 3 x PG4
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: I222
Unit cell:
a: 55.62Å b: 83.23Å c: 120.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.134 0.133 0.18
Expression system: Escherichia coli

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