› 3l7k

X-ray diffraction
3.1Å resolution

Structure of the Wall Teichoic Acid Polymerase TagF, H444N + CDPG (15 minute soak)

Released:
DOI: 10.2210/pdb3l7k/pdb
PDB entry 3l7k coloured by chain, front view.
PDB entry 3l7k coloured by chain, side view.
PDB entry 3l7k coloured by chain, top view.
Source organism: Staphylococcus epidermidis RP62A
Primary publication:
Structure of the bacterial teichoic acid polymerase TagF provides insights into membrane association and catalysis.
Lovering AL, Lin LY, Sewell EW, Spreter T, Brown ED, Strynadka NC
Nat Struct Mol Biol 17 582-9 (2010)
PMID: 20400947

Function and Biology Details

Reaction catalysed: 
n CDP-glycerol + 4-O-((2R)-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = n CMP + 4-O-(((2R)-glycerophospho)n-(2R)-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177227 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Teichoic acid poly(glycerol phosphate) polymerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 729 amino acids
Theoretical weight: 87.01 KDa
Source organism: Staphylococcus epidermidis RP62A
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5HLM5 (Residues: 1-721; Coverage: 100%)
Gene names: SERP1960, tagF
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand SO4 6 x SO4
Ligand CL 22 x CL
Ligand EDT 2 x EDT
Ligand C2G 1 x C2G
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P41212
Unit cell:
a: 222.04Å b: 222.04Å c: 100.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.19 0.252
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Glycosyltransferase structural biology and its role in the design of catalysts for glycosylation.
Chang et al. (2011)
7 more

1 mention without citation

A multi-enzyme machine polymerizes the Haemophilus influenzae type b capsule.
Cifuente et al. (2023)