› 3l8k

X-ray diffraction
2.5Å resolution

Crystal structure of a dihydrolipoyl dehydrogenase from Sulfolobus solfataricus

Released:
DOI: 10.2210/pdb3l8k/pdb
PDB entry 3l8k coloured by chain, front view.
PDB entry 3l8k coloured by chain, side view.
PDB entry 3l8k coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Entry authors: Bonanno JB, Rutter M, Bain KT, Miller S, Sampathkumar P, Wasserman S, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Protein N(6)-(dihydrolipoyl)lysine + NAD(+) = protein N(6)-(lipoyl)lysine + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189034 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrolipoamide dehydrogenase (PdhD-3) Chains: A, B
Molecule details ›
Chains: A, B
Length: 466 amino acids
Theoretical weight: 51.77 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q97XZ3 (Residues: 2-456; Coverage: 100%)
Gene names: SSO1565, pdhD-3
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 2 x ADP
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: I212121
Unit cell:
a: 114.186Å b: 126.737Å c: 165.993Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.243
Expression system: Escherichia coli BL21(DE3)

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