› 3la2

X-ray diffraction
2.6Å resolution

Crystal structure of NtcA in complex with 2-oxoglutarate

Released:
DOI: 10.2210/pdb3la2/pdb
PDB entry 3la2 coloured by chain, front view.
PDB entry 3la2 coloured by chain, side view.
PDB entry 3la2 coloured by chain, top view.
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Primary publication:
Structural basis for the allosteric control of the global transcription factor NtcA by the nitrogen starvation signal 2-oxoglutarate.
Zhao MX, Jiang YL, He YX, Chen YF, Teng YB, Chen Y, Zhang CC, Zhou CZ
Proc Natl Acad Sci U S A 107 12487-92 (2010)
PMID: 20616047

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141267 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Global nitrogen regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 243 amino acids
Theoretical weight: 27.12 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A4U6 (Residues: 1-223; Coverage: 100%)
Gene names: alr4392, bifA, ntcA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand AKG 2 x AKG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 68.14Å b: 70.26Å c: 149.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.222 0.268
Expression system: Escherichia coli

Quick links

Citations

14 review citations

The Emergence of 2-Oxoglutarate as a Master Regulator Metabolite.
Huergo et al. (2015)
13 more

2 mentions without citation

Structural basis for the allosteric control of the global transcription factor NtcA by the nitrogen starvation signal 2-oxoglutarate.
Zhao et al. (2010)
1 more