› 3la4

X-ray diffraction
2.05Å resolution

Crystal structure of the first plant urease from Jack bean (Canavalia ensiformis)

Released:
DOI: 10.2210/pdb3la4/pdb
PDB entry 3la4 coloured by chain, front view.
PDB entry 3la4 coloured by chain, side view.
PDB entry 3la4 coloured by chain, top view.
Source organism: Canavalia ensiformis
Primary publication:
Crystal structure of the first plant urease from jack bean: 83 years of journey from its first crystal to molecular structure.
Balasubramanian A, Ponnuraj K
J Mol Biol 400 274-83 (2010)
PMID: 20471401

Function and Biology Details

Reaction catalysed: 
Urea + H(2)O = CO(2) + 2 NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139572 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Urease Chain: A
Molecule details ›
Chain: A
Length: 840 amino acids
Theoretical weight: 91.13 KDa
Source organism: Canavalia ensiformis
UniProt:
  • Canonical: P07374 (Residues: 1-840; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NI 2 x NI
Ligand PO4 2 x PO4
Ligand ACN 1 x ACN
2 modified residues:
Ligand CME 3 x CME
Ligand KCX 1 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P6322
Unit cell:
a: 138.565Å b: 138.565Å c: 198.355Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.183 0.201

Quick links

Citations

12 review citations

Nickel-dependent metalloenzymes.
Boer et al. (2014)
11 more

41 mentions without citation

Nonredox nickel enzymes.
Maroney et al. (2014)
40 more