› 3lah

X-ray diffraction
2Å resolution

Structural insights into the molecular mechanism of H-NOX activation

Released:
DOI: 10.2210/pdb3lah/pdb
PDB entry 3lah coloured by chain, front view.
PDB entry 3lah coloured by chain, side view.
PDB entry 3lah coloured by chain, top view.
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Primary publication:
Structural insights into the molecular mechanism of H-NOX activation.
Olea C, Herzik MA, Kuriyan J, Marletta MA
Protein Sci 19 881-7 (2010)
PMID: 20162612

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185951 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyl-accepting transducer domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 188 amino acids
Theoretical weight: 21.97 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RBX6 (Residues: 1-188; Coverage: 31%)
Gene names: TTE0680, Tar4
Sequence domains: Haem-NO-binding
Structure domains: H-NOX domain

Ligands and Environments

2 bound ligands:
Ligand HEM 2 x HEM
Ligand IMD 4 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P212121
Unit cell:
a: 61.353Å b: 88.713Å c: 89.207Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.215 0.251
Expression system: Escherichia coli

Quick links

Citations

8 review citations

The chemistry and biology of soluble guanylate cyclase stimulators and activators.
Follmann et al. (2013)
7 more

1 mention without citation

Structural insights into the molecular mechanism of H-NOX activation.
Olea et al. (2010)