› 3laj

X-ray diffraction
2.31Å resolution

The Structure of the Intermediate Complex of the Arginine Repressor from Mycobacterium tuberculosis Bound to its DNA Operator and L-arginine.

Released:
DOI: 10.2210/pdb3laj/pdb
PDB entry 3laj coloured by chain, front view.
PDB entry 3laj coloured by chain, side view.
PDB entry 3laj coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Crystal structure of the intermediate complex of the arginine repressor from Mycobacterium tuberculosis bound with its DNA operator reveals detailed mechanism of arginine repression.
Cherney LT, Cherney MM, Garen CR, James MN
J Mol Biol 399 240-54 (2010)
PMID: 20382162

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116524 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Arginine repressor Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 170 amino acids
Theoretical weight: 17.37 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WPY9 (Residues: 1-170; Coverage: 100%)
Gene names: MTCY06H11.22, Rv1657, ahrC, argR
Sequence domains:
Structure domains:
5'-D(*TP*TP*GP*CP*AP*TP*AP*AP*CP*GP*AP*TP*GP*CP*AP*A)-3' Chains: G, I, K
Molecule details ›
Chains: G, I, K
Length: 16 nucleotides
Theoretical weight: 4.91 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Not provided
5'-D(*TP*TP*GP*CP*AP*TP*CP*GP*TP*TP*AP*TP*GP*CP*AP*A)-3' Chains: H, J, L
Molecule details ›
Chains: H, J, L
Length: 16 nucleotides
Theoretical weight: 4.89 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ARG 6 x ARG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 185.686Å b: 106.788Å c: 119.08Å
α: 90° β: 121.75° γ: 90°
R-values:
R R work R free
0.185 0.184 0.207
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

Transcription factor-based biosensors enlightened by the analyte.
Fernandez-López et al. (2015)
4 other articles

3 mentions without citation

Conserved Dynamic Mechanism of Allosteric Response to L-arg in Divergent Bacterial Arginine Repressors.
Pandey et al. (2020)
2 more