› 3lez

X-ray diffraction
1.25Å resolution

Crystal structure of a halotolerant bacterial beta-lactamase

Released:
DOI: 10.2210/pdb3lez/pdb
PDB entry 3lez coloured by chain, front view.
PDB entry 3lez coloured by chain, side view.
PDB entry 3lez coloured by chain, top view.
Source organism: Oceanobacillus iheyensis
Primary publication:
An antibiotic-resistance enzyme from a deep-sea bacterium.
Toth M, Smith C, Frase H, Mobashery S, Vakulenko S
J Am Chem Soc 132 816-23 (2010)
PMID: 20000704

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-184427 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 28.87 KDa
Source organism: Oceanobacillus iheyensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8EMP8 (Residues: 45-304; Coverage: 86%)
Gene name: OB2793
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

3 bound ligands:
Ligand CA 8 x CA
Ligand CL 1 x CL
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P3121
Unit cell:
a: 55.98Å b: 55.98Å c: 165.89Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.155 0.154 0.174
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Epidemiological expansion, structural studies, and clinical challenges of new β-lactamases from gram-negative bacteria.
Bush et al. (2011)
4 more