› 3lf5

X-ray diffraction
1.25Å resolution

Structure of Human NADH cytochrome b5 oxidoreductase (Ncb5or) b5 Domain to 1.25A Resolution

Released:
DOI: 10.2210/pdb3lf5/pdb
PDB entry 3lf5 coloured by chain, front view.
PDB entry 3lf5 coloured by chain, side view.
PDB entry 3lf5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Study of the individual cytochrome b5 and cytochrome b5 reductase domains of Ncb5or reveals a unique heme pocket and a possible role of the CS domain.
Deng B, Parthasarathy S, Wang W, Gibney BR, Battaile KP, Lovell S, Benson DR, Zhu H
J Biol Chem 285 30181-91 (2010)
PMID: 20630863

Function and Biology Details

Reaction catalysed: 
NADH + 2 ferricytochrome b5 = NAD(+) + H(+) + 2 ferrocytochrome b5
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181621 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome b5 reductase 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 88 amino acids
Theoretical weight: 10.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7L1T6 (Residues: 50-137; Coverage: 17%)
Gene names: CYB5R4, NCB5OR
Sequence domains: Cytochrome b5-like Heme/Steroid binding domain
Structure domains: Cytochrome b5-like heme/steroid binding domain

Ligands and Environments

2 bound ligands:
Ligand HEM 2 x HEM
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-BM
Spacegroup: P21
Unit cell:
a: 38.48Å b: 56.37Å c: 42.56Å
α: 90° β: 100.67° γ: 90°
R-values:
R R work R free
0.148 0.147 0.173
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Lipotoxicity in the liver.
Zámbó et al. (2013)
4 more

4 mentions without citation

Advances in the Understanding of Protein-Protein Interactions in Drug Metabolizing Enzymes through the Use of Biophysical Techniques.
Lampe JN. (2017)
3 more