› 3lfu

X-ray diffraction
1.8Å resolution

Crystal Structure of E. coli UvrD

Released:
DOI: 10.2210/pdb3lfu/pdb
PDB entry 3lfu coloured by chain, front view.
PDB entry 3lfu coloured by chain, side view.
PDB entry 3lfu coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Rotations of the 2B sub-domain of E. coli UvrD helicase/translocase coupled to nucleotide and DNA binding.
Jia H, Korolev S, Niedziela-Majka A, Maluf NK, Gauss GH, Myong S, Ha T, Waksman G, Lohman TM
J Mol Biol 411 633-48 (2011)
PMID: 21704638

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136462 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA helicase II Chain: A
Molecule details ›
Chain: A
Length: 647 amino acids
Theoretical weight: 74.08 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03018 (Residues: 1-647; Coverage: 90%)
Gene names: JW3786, b3813, mutU, pdeB, rad, recL, uvrD
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 194.543Å b: 58.739Å c: 69.418Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.199 0.248
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Single-molecule views of protein movement on single-stranded DNA.
Ha et al. (2012)
6 more

13 mentions without citation

Protein structure. Direct observation of structure-function relationship in a nucleic acid-processing enzyme.
Comstock et al. (2015)
12 more