› 3lfy

X-ray diffraction
2.6Å resolution

CTD of Tarocystatin in complex with papain

Released:
DOI: 10.2210/pdb3lfy/pdb
PDB entry 3lfy coloured by chain, front view.
PDB entry 3lfy coloured by chain, side view.
PDB entry 3lfy coloured by chain, top view.
Source organism: Carica papaya
Primary publication:
Crystal structure of tarocystatin-papain complex: implications for the inhibition property of group-2 phytocystatins.
Chu MH, Liu KL, Wu HY, Yeh KW, Cheng YS
Planta 234 243-54 (2011)
PMID: 21416241

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins with broad specificity for peptide bonds, but preference for an amino acid bearing a large hydrophobic side chain at the P2 position. Does not accept Val in P1'.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133539 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Papain Chains: A, C
Molecule details ›
Chains: A, C
Length: 212 amino acids
Theoretical weight: 23.5 KDa
Source organism: Carica papaya
UniProt:
  • Canonical: P00784 (Residues: 134-345; Coverage: 65%)
Sequence domains: Papain family cysteine protease
Structure domains: Cysteine proteinases

Ligands and Environments

3 bound ligands:
Ligand SER 2 x SER
Ligand ASN 2 x ASN
Ligand GLY 1 x GLY
1 modified residue:
Ligand OCS 2 x OCS

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: P31
Unit cell:
a: 48.89Å b: 48.89Å c: 200.87Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.213 0.213 0.267

Quick links

Citations

3 review citations

C1A cysteine protease-cystatin interactions in leaf senescence.
Díaz-Mendoza et al. (2014)
2 more

3 mentions without citation

Mechanisms Applied by Protein Inhibitors to Inhibit Cysteine Proteases.
Tušar et al. (2021)
2 more