› 3lgi

X-ray diffraction
1.65Å resolution

Structure of the protease domain of DegS (DegS-deltaPDZ) at 1.65 A

Released:
DOI: 10.2210/pdb3lgi/pdb
PDB entry 3lgi coloured by chain, front view.
PDB entry 3lgi coloured by chain, side view.
PDB entry 3lgi coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Allostery is an intrinsic property of the protease domain of DegS: implications for enzyme function and evolution.
Sohn J, Grant RA, Sauer RT
J Biol Chem 285 34039-47 (2010)
PMID: 20739286

Function and Biology Details

Reaction catalysed: 
Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142438 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine endoprotease DegS Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 237 amino acids
Theoretical weight: 25.53 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEE3 (Residues: 26-256; Coverage: 65%)
Gene names: JW3204, b3235, degS, hhoB, htrH
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 70.384Å b: 72.762Å c: 128.877Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.152 0.182
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Membrane proteases in the bacterial protein secretion and quality control pathway.
Dalbey et al. (2012)
1 more

1 mention without citation

Allostery is an intrinsic property of the protease domain of DegS: implications for enzyme function and evolution.
Sohn et al. (2010)