› 3lhc

X-ray diffraction
1.34Å resolution

Crystal structure of cyanovirin-n swapping domain b mutant

Released:
DOI: 10.2210/pdb3lhc/pdb
PDB entry 3lhc coloured by chain, front view.
PDB entry 3lhc coloured by chain, side view.
PDB entry 3lhc coloured by chain, top view.
Source organism: Nostoc ellipsosporum
Primary publication:
Anti-HIV activity of defective cyanovirin-N mutants is restored by dimerization.
Matei E, Zheng A, Furey W, Rose J, Aiken C, Gronenborn AM
J Biol Chem 285 13057-65 (2010)
PMID: 20147291

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160490 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyanovirin-N Chain: A
Molecule details ›
Chain: A
Length: 100 amino acids
Theoretical weight: 10.61 KDa
Source organism: Nostoc ellipsosporum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P81180 (Residues: 1-101; Coverage: 99%)
Sequence domains: CVNH domain
Structure domains: Cyanovirin-N

Ligands and Environments

2 bound ligands:
Ligand NA 2 x NA
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P3221
Unit cell:
a: 47.14Å b: 47.14Å c: 78.39Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.199 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Cyanobacterial lectins characteristics and their role as antiviral agents.
Singh et al. (2017)
25 other articles

1 mention without citation

Anti-HIV activity of defective cyanovirin-N mutants is restored by dimerization.
Matei et al. (2010)