› 3lia

X-ray diffraction
1.99Å resolution

Crystal Structure of the extracellular domain of the putative histidine kinase mmHK1S-Z2

Released:
DOI: 10.2210/pdb3lia/pdb
PDB entry 3lia coloured by chain, front view.
PDB entry 3lia coloured by chain, side view.
PDB entry 3lia coloured by chain, top view.
Source organism: Methanosarcina mazei
Primary publication:
Structural characterization of the predominant family of histidine kinase sensor domains.
Zhang Z, Hendrickson WA
J Mol Biol 400 335-53 (2010)
PMID: 20435045

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-185799 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 291 amino acids
Theoretical weight: 32.7 KDa
Source organism: Methanosarcina mazei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8PSW8 (Residues: 32-313; Coverage: 32%)
Gene name: MM_2955
Sequence domains: Cache domain
Structure domains: PAS domain

Ligands and Environments

2 bound ligands:
Ligand BTB 2 x BTB
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 46.525Å b: 98.494Å c: 71.04Å
α: 90° β: 87.51° γ: 90°
R-values:
R R work R free
0.182 0.178 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Evolution of two-component signal transduction systems.
Capra et al. (2012)
18 more

6 mentions without citation

Diversity in Sensing and Signaling of Bacterial Sensor Histidine Kinases.
Ishii et al. (2021)
5 more