› 3lie

X-ray diffraction
2.59Å resolution

Crystal Structure of the extracellular domain of the putative histidine kinase vpHK1S-Z8

Released:
DOI: 10.2210/pdb3lie/pdb
PDB entry 3lie coloured by chain, front view.
PDB entry 3lie coloured by chain, side view.
PDB entry 3lie coloured by chain, top view.
Source organism: Vibrio parahaemolyticus
Primary publication:
Structural characterization of the predominant family of histidine kinase sensor domains.
Zhang Z, Hendrickson WA
J Mol Biol 400 335-53 (2010)
PMID: 20435045

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183374 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative sensory box/GGDEF family protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 295 amino acids
Theoretical weight: 34.26 KDa
Source organism: Vibrio parahaemolyticus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q87SR8 (Residues: 28-313; Coverage: 47%)
Gene name: VP0354
Sequence domains: Bacterial histidine kinase, sensor domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21212
Unit cell:
a: 78.049Å b: 114.953Å c: 69.624Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.258 0.254 0.326
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Evolution of two-component signal transduction systems.
Capra et al. (2012)
18 more

3 mentions without citation

Diversity in Sensing and Signaling of Bacterial Sensor Histidine Kinases.
Ishii et al. (2021)
2 more