› 3lki

X-ray diffraction
2.04Å resolution

Crystal Structure of Fructokinase with bound ATP from Xylella fastidiosa

Released:
DOI: 10.2210/pdb3lki/pdb
PDB entry 3lki coloured by chain, front view.
PDB entry 3lki coloured by chain, side view.
PDB entry 3lki coloured by chain, top view.
Source organism: Xylella fastidiosa Temecula1
Entry authors: Satyanarayana L, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
ATP + D-fructose = ADP + D-fructose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183301 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbohydrate kinase PfkB domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 338 amino acids
Theoretical weight: 36.82 KDa
Source organism: Xylella fastidiosa Temecula1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q87CC0 (Residues: 12-338; Coverage: 97%)
Gene name: PD_1163
Sequence domains: pfkB family carbohydrate kinase
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments

3 bound ligands:
Ligand K 1 x K
Ligand PO4 1 x PO4
Ligand ATP 1 x ATP
1 modified residue:
Ligand MSE 22 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 70.221Å b: 92.651Å c: 179.765Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.222
Expression system: Escherichia coli BL21(DE3)

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