› 3lkx

X-ray diffraction
2.5Å resolution

Human nac dimerization domain

Released:
DOI: 10.2210/pdb3lkx/pdb
PDB entry 3lkx coloured by chain, front view.
PDB entry 3lkx coloured by chain, side view.
PDB entry 3lkx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of the human nascent polypeptide-associated complex domain reveals a nucleic acid-binding region on the NACA subunit .
Liu Y, Hu Y, Li X, Niu L, Teng M
Biochemistry 49 2890-6 (2010)
PMID: 20214399

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148926 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transcription factor BTF3 Chain: A
Molecule details ›
Chain: A
Length: 66 amino acids
Theoretical weight: 7.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20290 (Residues: 97-162; Coverage: 32%)
Gene names: BTF3, NACB, OK/SW-cl.8
Sequence domains: NAC domain
Structure domains: Nascent polypeptide-associated complex domain
Nascent polypeptide-associated complex subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 54 amino acids
Theoretical weight: 6.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13765 (Residues: 84-136; Coverage: 25%)
Gene names: HSD48, NACA
Sequence domains: NAC domain
Structure domains: Nascent polypeptide-associated complex domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 3W1A
Spacegroup: P6122
Unit cell:
a: 59.755Å b: 59.755Å c: 176.823Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.215 0.253
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Chaperone Interactions at the Ribosome.
Deuerling et al. (2019)
1 more

1 mention without citation

Conformational flexibility within the nascent polypeptide-associated complex enables its interactions with structurally diverse client proteins.
Martin et al. (2018)