› 3lms

X-ray diffraction
2.5Å resolution

Structure of human activated thrombin-activatable fibrinolysis inhibitor, TAFIa, in complex with tick-derived funnelin inhibitor, TCI.

Released:
DOI: 10.2210/pdb3lms/pdb
PDB entry 3lms coloured by chain, front view.
PDB entry 3lms coloured by chain, side view.
PDB entry 3lms coloured by chain, top view.
Source organisms:
Primary publication:
Insights into the molecular inactivation mechanism of human activated thrombin-activatable fibrinolysis inhibitor.
Sanglas L, Arolas JL, Valnickova Z, Aviles FX, Enghild JJ, Gomis-Rüth FX
J Thromb Haemost 8 1056-65 (2010)
PMID: 20088943

Function and Biology Details

Reaction catalysed: 
Release of C-terminal Arg and Lys from a polypeptide.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177033 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Carboxypeptidase B2 Chain: A
Molecule details ›
Chain: A
Length: 309 amino acids
Theoretical weight: 35.84 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q96IY4 (Residues: 115-423; Coverage: 77%)
Gene name: CPB2
Sequence domains: Zinc carboxypeptidase
Structure domains: Zn peptidases
Carboxypeptidase inhibitor Chain: B
Molecule details ›
Chain: B
Length: 74 amino acids
Theoretical weight: 7.81 KDa
Source organism: Rhipicephalus bursa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5EPH2 (Residues: 23-96; Coverage: 99%)
Sequence domains: Carboxypeptidase inhibitor I68
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand ZN 1 x ZN
Ligand GLY 1 x GLY
Ligand GOL 4 x GOL
Ligand CL 3 x CL
Ligand CA 1 x CA
Ligand K 2 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P3121
Unit cell:
a: 157.18Å b: 157.18Å c: 57.07Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.185 0.216
Expression system: Escherichia coli

Quick links

Citations

2 review citations

New antithrombotic drugs: Antithrombotic Therapy and Prevention of Thrombosis, 9th ed: American College of Chest Physicians Evidence-Based Clinical Practice Guidelines.
Weitz et al. (2012)
1 more

4 mentions without citation

Thrombin Activatable Fibrinolysis Inhibitor (TAFI): An Updated Narrative Review.
Sillen et al. (2021)
3 more