› 3lnq

X-ray diffraction
2.25Å resolution

Structure of Aristaless homeodomain in complex with DNA

Released:
DOI: 10.2210/pdb3lnq/pdb
PDB entry 3lnq coloured by chain, front view.
PDB entry 3lnq coloured by chain, side view.
PDB entry 3lnq coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Cooperative DNA-binding and sequence-recognition mechanism of aristaless and clawless.
Miyazono K, Zhi Y, Takamura Y, Nagata K, Saigo K, Kojima T, Tanokura M
EMBO J 29 1613-23 (2010)
PMID: 20389279

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116531 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Homeobox protein aristaless Chain: A
Molecule details ›
Chain: A
Length: 58 amino acids
Theoretical weight: 7.24 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06453 (Residues: 87-144; Coverage: 14%)
Gene names: CG3935, al
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
5'-D(*GP*GP*GP*TP*TP*TP*AP*AP*TP*TP*AP*GP*GP*G)-3' Chain: B
Molecule details ›
Chain: B
Length: 14 nucleotides
Theoretical weight: 4.39 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
5'-D(*CP*CP*CP*TP*AP*AP*TP*TP*AP*AP*AP*CP*CP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 14 nucleotides
Theoretical weight: 4.17 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P43212
Unit cell:
a: 42.35Å b: 42.35Å c: 162.73Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.227 0.251
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Investigating Developmental and Epileptic Encephalopathy Using Drosophila melanogaster.
Takai et al. (2020)
18 other articles

13 mentions without citation

Unmet care needs of advanced cancer patients and their informal caregivers: a systematic review.
Wang et al. (2018)
12 more