› 3lom

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF GERANYLTRANSFERASE FROM Legionella pneumophila

Released:
DOI: 10.2210/pdb3lom/pdb
PDB entry 3lom coloured by chain, front view.
PDB entry 3lom coloured by chain, side view.
PDB entry 3lom coloured by chain, top view.
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Primary publication:
Prediction of function for the polyprenyl transferase subgroup in the isoprenoid synthase superfamily.
Wallrapp FH, Pan JJ, Ramamoorthy G, Almonacid DE, Hillerich BS, Seidel R, Patskovsky Y, Babbitt PC, Almo SC, Jacobson MP, Poulter CD
Proc Natl Acad Sci U S A 110 E1196-202 (2013)
PMID: 23493556

Function and Biology Details

Reaction catalysed: 
Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178613 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Geranyltranstransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 313 amino acids
Theoretical weight: 34.78 KDa
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5ZT35 (Residues: 7-309; Coverage: 98%)
Gene names: ispA, lpg2330
Sequence domains: Polyprenyl synthetase
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

1 bound ligand:
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P43212
Unit cell:
a: 94.775Å b: 94.775Å c: 148.345Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.221 0.267
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Enzyme Function Initiative-Enzyme Similarity Tool (EFI-EST): A web tool for generating protein sequence similarity networks.
Gerlt et al. (2015)
14 more

1 mention without citation

Prediction of function for the polyprenyl transferase subgroup in the isoprenoid synthase superfamily.
Wallrapp et al. (2013)