› 3lou

X-ray diffraction
1.9Å resolution

Crystal structure of Formyltetrahydrofolate deformylase (YP_105254.1) from BURKHOLDERIA MALLEI ATCC 23344 at 1.90 A resolution

Released:
DOI: 10.2210/pdb3lou/pdb
PDB entry 3lou coloured by chain, front view.
PDB entry 3lou coloured by chain, side view.
PDB entry 3lou coloured by chain, top view.
Source organism: Burkholderia mallei ATCC 23344
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
10-formyltetrahydrofolate + H(2)O = formate + tetrahydrofolate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101590 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formyltetrahydrofolate deformylase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 292 amino acids
Theoretical weight: 33.18 KDa
Source organism: Burkholderia mallei ATCC 23344
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2WCF8 (Residues: 1-291; Coverage: 100%)
Gene names: BMAA0482, purU, purU-2
Sequence domains: Formyl transferase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand UNL 1 x UNL
Ligand SO4 12 x SO4
Ligand EDO 23 x EDO
Ligand PEG 7 x PEG
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 74.628Å b: 93.476Å c: 98.802Å
α: 90° β: 95.29° γ: 90°
R-values:
R R work R free
0.187 0.185 0.221
Expression system: Escherichia coli

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