› 3lq0

X-ray diffraction
1.45Å resolution

Zymogen structure of crayfish astacin metallopeptidase

Released:
DOI: 10.2210/pdb3lq0/pdb
PDB entry 3lq0 coloured by chain, front view.
PDB entry 3lq0 coloured by chain, side view.
PDB entry 3lq0 coloured by chain, top view.
Source organism: Astacus astacus
Primary publication:
Proenzyme structure and activation of astacin metallopeptidase.
Guevara T, Yiallouros I, Kappelhoff R, Bissdorf S, Stöcker W, Gomis-Rüth FX
J Biol Chem 285 13958-65 (2010)
PMID: 20202938

Function and Biology Details

Reaction catalysed: 
Hydrolysis of peptide bonds in substrates containing five or more amino acids, preferentially with Ala in P1', and Pro in P2'.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139644 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Astacin Chain: A
Molecule details ›
Chain: A
Length: 235 amino acids
Theoretical weight: 26.44 KDa
Source organism: Astacus astacus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07584 (Residues: 16-250; Coverage: 100%)
Sequence domains: Astacin (Peptidase family M12A)
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand GOL 3 x GOL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 55.91Å b: 63.37Å c: 69.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.152 0.18
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Domain structure and function of matrix metalloprotease 23 (MMP23): role in potassium channel trafficking.
Galea et al. (2014)
1 more

10 mentions without citation

Architecture and function of metallopeptidase catalytic domains.
Cerdà-Costa et al. (2014)
9 more