› 3lq1

X-ray diffraction
2.6Å resolution

Crystal structure of 2-succinyl-6-hydroxy-2,4-cyclohexadiene 1-carboxylic acid synthase/2-oxoglutarate decarboxylase FROM Listeria monocytogenes str. 4b F2365

Released:
DOI: 10.2210/pdb3lq1/pdb
PDB entry 3lq1 coloured by chain, front view.
PDB entry 3lq1 coloured by chain, side view.
PDB entry 3lq1 coloured by chain, top view.
Source organism: Listeria monocytogenes serotype 4b str. F2365
Entry authors: Patskovsky Y, Toro R, Freeman J, Hu S, Sauder JM, Burley SK, Almo SC, New York Structural GenomiX Research Consortium (NYSGXRC), New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-180770 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 578 amino acids
Theoretical weight: 64.55 KDa
Source organism: Listeria monocytogenes serotype 4b str. F2365
Expression system: Escherichia coli
UniProt:
  • Canonical: Q71YZ2 (Residues: 2-568; Coverage: 98%)
Gene names: LMOf2365_1699, menD
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P4322
Unit cell:
a: 99.695Å b: 99.695Å c: 259.982Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.222 0.265
Expression system: Escherichia coli

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