› 3lqq

X-ray diffraction
3.53Å resolution

Structure of the CED-4 Apoptosome

Released:
DOI: 10.2210/pdb3lqq/pdb
PDB entry 3lqq coloured by chain, front view.
PDB entry 3lqq coloured by chain, side view.
PDB entry 3lqq coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Crystal structure of the Caenorhabditis elegans apoptosome reveals an octameric assembly of CED-4.
Qi S, Pang Y, Hu Q, Liu Q, Li H, Zhou Y, He T, Liang Q, Liu Y, Yuan X, Luo G, Li H, Wang J, Yan N, Shi Y
Cell 141 446-57 (2010)
PMID: 20434985

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151742 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell death protein 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 549 amino acids
Theoretical weight: 62.95 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30429 (Residues: 1-571; Coverage: 96%)
Gene names: C35D10.9, ced-4
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: I422
Unit cell:
a: 181.64Å b: 181.64Å c: 203.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.277 0.275 0.311
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

29 review citations

Activation and regulation of the inflammasomes.
Latz et al. (2013)
28 more

10 mentions without citation

New insights into apoptosome structure and function.
Dorstyn et al. (2018)
9 more