› 3lrg

X-ray diffraction
2.05Å resolution

Structure of anti-huntingtin VL domain

Released:
DOI: 10.2210/pdb3lrg/pdb
PDB entry 3lrg coloured by chain, front view.
PDB entry 3lrg coloured by chain, side view.
PDB entry 3lrg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A disulfide-free single-domain V(L) intrabody with blocking activity towards huntingtin reveals a novel mode of epitope recognition.
Schiefner A, Chatwell L, Körner J, Neumaier I, Colby DW, Volkmer R, Wittrup KD, Skerra A
J Mol Biol 414 337-55 (2011)
PMID: 21968397

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213315 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
anti-huntingtin VL domain Chains: A, B
Molecule details ›
Chains: A, B
Length: 121 amino acids
Theoretical weight: 12.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand TRS 1 x TRS
Ligand IMD 1 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P41212
Unit cell:
a: 107.68Å b: 107.68Å c: 50.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.197
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Physical chemistry of polyglutamine: intriguing tales of a monotonous sequence.
Wetzel R. (2012)
6 more