› 3lrh

X-ray diffraction
2.6Å resolution

Structure of anti-huntingtin VL domain in complex with huntingtin peptide

Released:
DOI: 10.2210/pdb3lrh/pdb
PDB entry 3lrh coloured by chain, front view.
PDB entry 3lrh coloured by chain, side view.
PDB entry 3lrh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A disulfide-free single-domain V(L) intrabody with blocking activity towards huntingtin reveals a novel mode of epitope recognition.
Schiefner A, Chatwell L, Körner J, Neumaier I, Colby DW, Volkmer R, Wittrup KD, Skerra A
J Mol Biol 414 337-55 (2011)
PMID: 21968397

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211209 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
anti-huntingtin VL domain Chains: A, C, E, G, I, K, M, O
Molecule details ›
Chains: A, C, E, G, I, K, M, O
Length: 121 amino acids
Theoretical weight: 12.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Huntingtin Chains: B, D, F, H, J, L, N, P
Molecule details ›
Chains: B, D, F, H, J, L, N, P
Length: 14 amino acids
Theoretical weight: 1.69 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P42858 (Residues: 5-18; Coverage: 0%)
Gene names: HD, HTT, IT15

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 53.09Å b: 89.66Å c: 95Å
α: 90° β: 99.45° γ: 90°
R-values:
R R work R free
0.219 0.216 0.278
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Physical chemistry of polyglutamine: intriguing tales of a monotonous sequence.
Wetzel R. (2012)
6 more

4 mentions without citation

Structural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization.
Kato et al. (2016)
3 more