› 3lrs

X-ray diffraction
2.37Å resolution

Structure of PG16, an antibody with broad and potent neutralization of HIV-1

Released:
DOI: 10.2210/pdb3lrs/pdb
PDB entry 3lrs coloured by chain, front view.
PDB entry 3lrs coloured by chain, side view.
PDB entry 3lrs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of PG16 and chimeric dissection with somatically related PG9: structure-function analysis of two quaternary-specific antibodies that effectively neutralize HIV-1.
Pancera M, McLellan JS, Wu X, Zhu J, Changela A, Schmidt SD, Yang Y, Zhou T, Phogat S, Mascola JR, Kwong PD
J Virol 84 8098-110 (2010)
PMID: 20538861

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213316 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PG-16 Heavy Chain Fab Chains: A, C, E, H
Molecule details ›
Chains: A, C, E, H
Length: 238 amino acids
Theoretical weight: 25.92 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
PG-16 Light Chain Fab Chains: B, D, F, L
Molecule details ›
Chains: B, D, F, L
Length: 211 amino acids
Theoretical weight: 22.16 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21
Unit cell:
a: 81.317Å b: 165.085Å c: 81.363Å
α: 90° β: 91.55° γ: 90°
R-values:
R R work R free
0.213 0.211 0.26
Expression system: Homo sapiens

Quick links

Citations

28 review citations

HIV-1 neutralizing antibodies: understanding nature's pathways.
Mascola et al. (2013)
27 more

3 mentions without citation

Crystal structure of PG16 and chimeric dissection with somatically related PG9: structure-function analysis of two quaternary-specific antibodies that effectively neutralize HIV-1.
Pancera et al. (2010)
2 more