› 3ls4

X-ray diffraction
2Å resolution

Crystal Structure of Anti-tetrahydrocannabinol Fab Fragment in Complex with THC

Released:
DOI: 10.2210/pdb3ls4/pdb
PDB entry 3ls4 coloured by chain, front view.
PDB entry 3ls4 coloured by chain, side view.
PDB entry 3ls4 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
A structural insight into the molecular recognition of a (-)-Delta9-tetrahydrocannabinol and the development of a sensitive, one-step, homogeneous immunocomplex-based assay for its detection.
Niemi MH, Turunen L, Pulli T, Nevanen TK, Höyhtyä M, Söderlund H, Rouvinen J, Takkinen K
J Mol Biol 400 803-14 (2010)
PMID: 20630472

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213317 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Light chain of antibody Fab fragment Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.35 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Heavy chain of antibody Fab fragment Chain: H
Molecule details ›
Chain: H
Length: 219 amino acids
Theoretical weight: 23.37 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand TCI 1 x TCI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P43212
Unit cell:
a: 122.76Å b: 122.76Å c: 73.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.213 0.261
Expression system: Escherichia coli

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Citations

1 citations in other articles

Structure-guided residence time optimization of a dabigatran reversal agent.
Schiele et al. (2015)

4 mentions without citation

Structural and Functional Characterization of Anti-A33 Antibodies Reveal a Potent Cross-Species Orthopoxviruses Neutralizer.
Matho et al. (2015)
3 more