› 3lsn

X-ray diffraction
1.35Å resolution

Crystal structure of putative geranyltranstransferase from PSEUDOMONAS fluorescens PF-5 complexed with magnesium

Released:
DOI: 10.2210/pdb3lsn/pdb
PDB entry 3lsn coloured by chain, front view.
PDB entry 3lsn coloured by chain, side view.
PDB entry 3lsn coloured by chain, top view.
Source organism: Pseudomonas protegens Pf-5
Entry authors: Malashkevich VN, Toro R, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175598 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Geranyltranstransferase Chain: A
Molecule details ›
Chain: A
Length: 304 amino acids
Theoretical weight: 32.91 KDa
Source organism: Pseudomonas protegens Pf-5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4K5A6 (Residues: 3-295; Coverage: 99%)
Gene names: PFL_5509, ispA
Sequence domains: Polyprenyl synthetase
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

1 bound ligand:
Ligand MG 3 x MG
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 84.182Å b: 47.875Å c: 75.982Å
α: 90° β: 120.78° γ: 90°
R-values:
R R work R free
0.18 0.179 0.201
Expression system: Escherichia coli BL21(DE3)

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