› 3lsr

X-ray diffraction
2.55Å resolution

Crystal structure of DesT in complex with duplex DNA

Released:
DOI: 10.2210/pdb3lsr/pdb
PDB entry 3lsr coloured by chain, front view.
PDB entry 3lsr coloured by chain, side view.
PDB entry 3lsr coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural basis for the transcriptional regulation of membrane lipid homeostasis.
Miller DJ, Zhang YM, Subramanian C, Rock CO, White SW
Nat Struct Mol Biol 17 971-5 (2010)
PMID: 20639888

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116534 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
HTH tetR-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 220 amino acids
Theoretical weight: 24 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HUS3 (Residues: 1-209; Coverage: 100%)
Gene names: PA4890, desT
Sequence domains: Bacterial regulatory proteins, tetR family
Structure domains:
DNA (27-MER) Chain: B
Molecule details ›
Chain: B
Length: 33 nucleotides
Theoretical weight: 10.15 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: I41
Unit cell:
a: 79.358Å b: 79.358Å c: 145.458Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.228 0.265
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Bacterial lipids: metabolism and membrane homeostasis.
Parsons et al. (2013)
8 more

8 mentions without citation

The TetR family of regulators.
Cuthbertson et al. (2013)
7 more