› 3lt8

X-ray diffraction
2.55Å resolution

A non-biological ATP binding protein with a single point mutation (D65V), that contributes to optimized folding and ligand binding, crystallized in the presence of 100 mM ATP.

Released:
DOI: 10.2210/pdb3lt8/pdb
PDB entry 3lt8 coloured by chain, front view.
PDB entry 3lt8 coloured by chain, side view.
PDB entry 3lt8 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Three-dimensional structures reveal multiple ADP/ATP binding modes for a synthetic class of artificial proteins.
Simmons CR, Magee CL, Smith DA, Lauman L, Chaput JC, Allen JP
Biochemistry 49 8689-99 (2010)
PMID: 20822107

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163753 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP BINDING PROTEIN-D65V Chain: A
Molecule details ›
Chain: A
Length: 81 amino acids
Theoretical weight: 9.71 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand ADP 1 x ADP
Ligand PEG 3 x PEG
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P3221
Unit cell:
a: 71.461Å b: 71.461Å c: 55.583Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.178 0.239
Expression system: Escherichia coli

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