› 3lti

X-ray diffraction
1.6Å resolution

Crystal structure of the Escherichia coli RNA polymerase beta subunit beta2-betai4 domains

Released:
DOI: 10.2210/pdb3lti/pdb
PDB entry 3lti coloured by chain, front view.
PDB entry 3lti coloured by chain, side view.
PDB entry 3lti coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Complete structural model of Escherichia coli RNA polymerase from a hybrid approach.
Opalka N, Brown J, Lane WJ, Twist KA, Landick R, Asturias FJ, Darst SA
PLoS Biol 8 (2010)
PMID: 20856905

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141748 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed RNA polymerase subunit beta Chain: A
Molecule details ›
Chain: A
Length: 296 amino acids
Theoretical weight: 34.64 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8V2 (Residues: 152-443; Coverage: 22%)
Gene names: JW3950, b3987, groN, nitB, rif, ron, rpoB, stl, stv, tabD
Sequence domains:
Structure domains: RNA polymerase Rpb2, domain 2

Ligands and Environments

No bound ligands
2 modified residues:
Ligand MSE 7 x MSE
Ligand MLY 10 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X3A
Spacegroup: P21212
Unit cell:
a: 106.314Å b: 52.039Å c: 61.826Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.208 0.23
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Unravelling the means to an end: RNA polymerase II transcription termination.
Kuehner et al. (2011)
6 more

4 mentions without citation

Structural biology of bacterial RNA polymerase.
Murakami KS. (2015)
3 more