› 3lub

X-ray diffraction
2.11Å resolution

Crystal structure of Putative creatinine amidohydrolase (YP_211512.1) from Bacteroides fragilis NCTC 9343 at 2.11 A resolution

Released:
DOI: 10.2210/pdb3lub/pdb
PDB entry 3lub coloured by chain, front view.
PDB entry 3lub coloured by chain, side view.
PDB entry 3lub coloured by chain, top view.
Source organism: Bacteroides fragilis NCTC 9343
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
Creatinine + H(2)O = creatine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177476 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative creatinine amidohydrolase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 254 amino acids
Theoretical weight: 28.54 KDa
Source organism: Bacteroides fragilis NCTC 9343
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5LE76 (Residues: 1-253; Coverage: 100%)
Gene name: BF9343_1795
Sequence domains: Creatinine amidohydrolase
Structure domains: Creatininase

Ligands and Environments

4 bound ligands:
Ligand ZN 24 x ZN
Ligand CA 12 x CA
Ligand CL 13 x CL
Ligand EDO 11 x EDO
1 modified residue:
Ligand MSE 84 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 55.906Å b: 156.79Å c: 170.161Å
α: 90° β: 95.29° γ: 90°
R-values:
R R work R free
0.153 0.151 0.193
Expression system: Escherichia coli

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