› 3lvd

X-ray diffraction
1.75Å resolution

Crystal structure of GFP-like protein aceGFP_G222E (A. coerulescens). UV-photoconverted green form.

Released:
DOI: 10.2210/pdb3lvd/pdb
Model geometry
Fit model/data
PDB entry 3lvd coloured by chain, front view.
PDB entry 3lvd coloured by chain, side view.
PDB entry 3lvd coloured by chain, top view.
Source organism: Aequorea coerulescens
Primary publication:
Structural evidence for a dehydrated intermediate in green fluorescent protein chromophore biosynthesis.
Pletneva NV, Pletnev VZ, Lukyanov KA, Gurskaya NG, Goryacheva EA, Martynov VI, Wlodawer A, Dauter Z, Pletnev S
J Biol Chem 285 15978-84 (2010)
PMID: 20220148

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180554 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 236 amino acids
Theoretical weight: 26.86 KDa
Source organism: Aequorea coerulescens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6YGZ0 (Residues: 1-238; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
1 modified residue:
Ligand GYS 2 x GYS

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 59.472Å b: 80.202Å c: 95.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.16 0.193
Expression system: Escherichia coli

Quick links

Citations

16 citation in other articles

Stabilizing role of glutamic acid 222 in the structure of Enhanced Green Fluorescent Protein.
Royant et al. (2011)
15 more

1 mention without citation

Structural evidence for a dehydrated intermediate in green fluorescent protein chromophore biosynthesis.
Pletneva et al. (2010)