› 3lvw

X-ray diffraction
2.5Å resolution

Glutathione-inhibited ScGCL

Released:
DOI: 10.2210/pdb3lvw/pdb
PDB entry 3lvw coloured by chain, front view.
PDB entry 3lvw coloured by chain, side view.
PDB entry 3lvw coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural basis for feedback and pharmacological inhibition of Saccharomyces cerevisiae glutamate cysteine ligase.
Biterova EI, Barycki JJ
J Biol Chem 285 14459-66 (2010)
PMID: 20220146

Function and Biology Details

Reaction catalysed: 
ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152324 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate--cysteine ligase Chain: A
Molecule details ›
Chain: A
Length: 692 amino acids
Theoretical weight: 79.93 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P32477 (Residues: 1-678; Coverage: 100%)
Gene names: GSH1, J0832, YJL101C
Sequence domains: Glutamate-cysteine ligase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GSH 1 x GSH
Ligand PGE 3 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P43212
Unit cell:
a: 118.127Å b: 118.127Å c: 165.799Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.199 0.251
Expression system: Escherichia coli

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Citations

2 review citations

Transporter engineering in biomass utilization by yeast.
Hara et al. (2017)
1 more

2 mentions without citation

Structural basis for feedback and pharmacological inhibition of Saccharomyces cerevisiae glutamate cysteine ligase.
Biterova et al. (2010)
1 more