› 3lw6

X-ray diffraction
1.81Å resolution

Crystal Structure of Drosophila beta1,4-galactosyltransferase-7

Released:
DOI: 10.2210/pdb3lw6/pdb
PDB entry 3lw6 coloured by chain, front view.
PDB entry 3lw6 coloured by chain, side view.
PDB entry 3lw6 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Crystal structure of the catalytic domain of Drosophila beta1,4-Galactosyltransferase-7.
Ramakrishnan B, Qasba PK
J Biol Chem 285 15619-15626 (2010)
PMID: 20236943

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-galactose + O-beta-D-xylosyl-[protein] = UDP + 4-beta-D-galactosyl-O-beta-D-xylosyl-[protein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194212 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-1,4-galactosyltransferase 7 Chain: A
Molecule details ›
Chain: A
Length: 287 amino acids
Theoretical weight: 33.32 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9VBZ9 (Residues: 71-311; Coverage: 75%)
Gene names: CG11780, beta-4GalT7, beta4Gal-T7, beta4GalT7, betaGalT7, dbeta4GalTI
Sequence domains:
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

3 bound ligands:
Ligand UDP 1 x UDP
Ligand MPD 1 x MPD
Ligand MN 1 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P43212
Unit cell:
a: 81.835Å b: 81.835Å c: 133.656Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.189 0.221
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Recent structures, evolution and mechanisms of glycosyltransferases.
Breton et al. (2012)
4 more

5 mentions without citation

Tick galactosyltransferases are involved in α-Gal synthesis and play a role during Anaplasma phagocytophilum infection and Ixodes scapularis tick vector development.
Cabezas-Cruz et al. (2018)
4 more