› 3lys

X-ray diffraction
2.8Å resolution

Crystal Structure of the N-terminal domain of the Prophage pi2 protein 01 (integrase) from Lactococcus lactis, Northeast Structural Genomics Consortium Target KR124F

Released:
DOI: 10.2210/pdb3lys/pdb
PDB entry 3lys coloured by chain, front view.
PDB entry 3lys coloured by chain, side view.
PDB entry 3lys coloured by chain, top view.
Source organism: Lactococcus lactis subsp. lactis
Entry authors: Forouhar F, Abashidze M, Seetharaman J, Sahdev S, Xiao R, Ciccosanti C, Belote RL, Everett JK, Nair R, Acton TB, Rost B, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189963 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Prophage pi2 protein 01, integrase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 112 amino acids
Theoretical weight: 13.44 KDa
Source organism: Lactococcus lactis subsp. lactis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9CGT4 (Residues: 53-156; Coverage: 28%)
Gene names: L36404, pi201
Sequence domains: Phage integrase, N-terminal SAM-like domain
Structure domains: DNA polymerase; domain 1

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P212121
Unit cell:
a: 66.704Å b: 90.272Å c: 156.948Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.26 0.26 0.29
Expression system: Escherichia coli BL21(DE3)

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