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› 3m12

X-ray diffraction
1.6Å resolution

Crystal Structure of the Lys265Arg phosphate-crytsallized mutant of monomeric sarcosine oxidase

Released:
DOI: 10.2210/pdb3m12/pdb
Model geometry
Fit model/data
PDB entry 3m12 coloured by chain, front view.
PDB entry 3m12 coloured by chain, side view.
PDB entry 3m12 coloured by chain, top view.
Source organism: Bacillus sp. B-0618
Primary publication:
Structural characterization of mutations at the oxygen activation site in monomeric sarcosine oxidase .
Jorns MS, Chen ZW, Mathews FS
Biochemistry 49 3631-9 (2010)
PMID: 20353187

Function and Biology Details

Reaction catalysed: 
sarcosine + O2 + H2O = formaldehyde + glycine + H2O2.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154199 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monomeric sarcosine oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 389 amino acids
Theoretical weight: 43.13 KDa
Source organism: Bacillus sp. B-0618
Expression system: Escherichia coli
UniProt:
  • Canonical: P40859 (Residues: 2-390; Coverage: 100%)
Gene names: sox, soxA
Sequence domains: FAD dependent oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21
Unit cell:
a: 72.323Å b: 69.251Å c: 73.232Å
α: 90° β: 93.68° γ: 90°
R-values:
R R work R free
0.181 0.179 0.215
Expression system: Escherichia coli

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Citations

13 citation in other articles

Structures and Mechanism of the Monoamine Oxidase Family.
Gaweska et al. (2011)
12 more