› 3m13

X-ray diffraction
2.1Å resolution

Crystal Structure of the Lys265Arg PEG-crystallized mutant of monomeric sarcosine oxidase

Released:
DOI: 10.2210/pdb3m13/pdb
PDB entry 3m13 coloured by chain, front view.
PDB entry 3m13 coloured by chain, side view.
PDB entry 3m13 coloured by chain, top view.
Source organism: Bacillus sp. B-0618
Primary publication:
Structural characterization of mutations at the oxygen activation site in monomeric sarcosine oxidase .
Jorns MS, Chen ZW, Mathews FS
Biochemistry 49 3631-9 (2010)
PMID: 20353187

Function and Biology Details

Reaction catalysed: 
Sarcosine + H(2)O + O(2) = glycine + formaldehyde + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154199 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monomeric sarcosine oxidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 389 amino acids
Theoretical weight: 43.13 KDa
Source organism: Bacillus sp. B-0618
Expression system: Escherichia coli
UniProt:
  • Canonical: P40859 (Residues: 2-390; Coverage: 100%)
Gene names: sox, soxA
Sequence domains: FAD dependent oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
2 bound ligands:
Ligand CL 4 x CL
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21
Unit cell:
a: 99.296Å b: 69.298Å c: 111.427Å
α: 90° β: 93.4° γ: 90°
R-values:
R R work R free
0.19 0.187 0.248
Expression system: Escherichia coli

Quick links

Citations

13 citation in other articles

Structures and Mechanism of the Monoamine Oxidase Family.
Gaweska et al. (2011)
12 more

1 mention without citation

Crystal structure of the bifunctional tRNA modification enzyme MnmC from Escherichia coli.
Kitamura et al. (2011)