› 3m1a

X-ray diffraction
2Å resolution

The Crystal Structure of a Short-chain Dehydrogenase from Streptomyces avermitilis to 2A

Released:
DOI: 10.2210/pdb3m1a/pdb
PDB entry 3m1a coloured by chain, front view.
PDB entry 3m1a coloured by chain, side view.
PDB entry 3m1a coloured by chain, top view.
Source organism: Streptomyces avermitilis
Entry authors: Stein AJ, Evdokimova E, Egorova O, Savchenko A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo decamer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182678 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dehydrogenase Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 281 amino acids
Theoretical weight: 29.83 KDa
Source organism: Streptomyces avermitilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q82PV2 (Residues: 1-281; Coverage: 100%)
Gene name: SAVERM_770
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand NA 4 x NA
Ligand ACT 2 x ACT
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 136.638Å b: 202.779Å c: 106.339Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.197 0.242
Expression system: Escherichia coli BL21(DE3)

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