› 3m24

X-ray diffraction
2.2Å resolution

Crystal structure of TagBFP fluorescent protein

Released:
DOI: 10.2210/pdb3m24/pdb
PDB entry 3m24 coloured by chain, front view.
PDB entry 3m24 coloured by chain, side view.
PDB entry 3m24 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Structural characterization of acylimine-containing blue and red chromophores in mTagBFP and TagRFP fluorescent proteins.
Subach OM, Malashkevich VN, Zencheck WD, Morozova KS, Piatkevich KD, Almo SC, Verkhusha VV
Chem Biol 17 333-41 (2010)
PMID: 20416505

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-163772 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TagBFP Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 232 amino acids
Theoretical weight: 26.42 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Green fluorescent protein

Ligands and Environments

3 bound ligands:
Ligand DTT 4 x DTT
Ligand GOL 7 x GOL
Ligand CL 2 x CL
1 modified residue:
Ligand NRP 4 x NRP

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 82.953Å b: 105.943Å c: 137.155Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.238
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
14 more

11 mentions without citation

Chromophore transformations in red fluorescent proteins.
Subach et al. (2012)
10 more