› 3m3n

X-ray diffraction
7Å resolution

Structure of a Longitudinal Actin Dimer Assembled by Tandem W Domains

Released:
DOI: 10.2210/pdb3m3n/pdb
PDB entry 3m3n coloured by chain, front view.
PDB entry 3m3n coloured by chain, side view.
PDB entry 3m3n coloured by chain, top view.
Source organisms:
Primary publication:
Structure of a longitudinal actin dimer assembled by tandem w domains: implications for actin filament nucleation.
Rebowski G, Namgoong S, Boczkowska M, Leavis PC, Navaza J, Dominguez R
J Mol Biol 403 11-23 (2010)
PMID: 20804767

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148835 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, alpha skeletal muscle Chains: A, B
Molecule details ›
Chains: A, B
Length: 375 amino acids
Theoretical weight: 41.88 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Actin nucleation-promoting factor WASL; Thymosin beta-4 Chain: W
Molecule details ›
Chain: W
Length: 101 amino acids
Theoretical weight: 11.09 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q91YD9 (Residues: 397-472; Coverage: 15%)
  • Canonical: P20065 (Residues: 24-50; Coverage: 54%)
Gene names: Ptmb4, Tmsb4, Tmsb4x, Wasl
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ATP 2 x ATP
Ligand CA 2 x CA
1 modified residue:
Ligand HIC 2 x HIC

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-BM
Spacegroup: P6522
Unit cell:
a: 100.743Å b: 100.743Å c: 458.839Å
α: 90° β: 90° γ: 120°
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Actin and Actin-Binding Proteins.
Pollard TD. (2016)
10 more

2 mentions without citation

Structure of a longitudinal actin dimer assembled by tandem w domains: implications for actin filament nucleation.
Rebowski et al. (2010)
1 more