› 3m3o

X-ray diffraction
2.1Å resolution

Crystal Structure of Agrocybe aegerita lectin AAL mutant R85A complexed with p-Nitrophenyl TF disaccharide

Released:
DOI: 10.2210/pdb3m3o/pdb
PDB entry 3m3o coloured by chain, front view.
PDB entry 3m3o coloured by chain, side view.
PDB entry 3m3o coloured by chain, top view.
Source organism: Cyclocybe aegerita
Primary publication:
Structural insights into the recognition mechanism between an antitumor galectin AAL and the Thomsen-Friedenreich antigen.
Feng L, Sun H, Zhang Y, Li DF, Wang DC
FASEB J 24 3861-8 (2010)
PMID: 20530247

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180509 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Anti-tumor lectin Chain: A
Molecule details ›
Chain: A
Length: 158 amino acids
Theoretical weight: 16.89 KDa
Source organism: Cyclocybe aegerita
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6WY08 (Residues: 1-158; Coverage: 100%)
Sequence domains: Galactoside-binding lectin
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: A2G, GAL
Carbohydrate polymer
2 bound ligands:
Ligand NPO 1 x NPO
Ligand BTB 1 x BTB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P3221
Unit cell:
a: 42.56Å b: 42.56Å c: 125.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.235 0.217 0.253
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Fungal lectins: structure, function and potential applications.
Varrot et al. (2013)
1 more

2 mentions without citation

Mushroom lectins: specificity, structure and bioactivity relevant to human disease.
Hassan et al. (2015)
1 more