› 3m3q

X-ray diffraction
2.2Å resolution

Crystal Structure of Agrocybe aegerita lectin AAL complexed with Ganglosides GM1 pentasaccharide

Released:
DOI: 10.2210/pdb3m3q/pdb
PDB entry 3m3q coloured by chain, front view.
PDB entry 3m3q coloured by chain, side view.
PDB entry 3m3q coloured by chain, top view.
Source organism: Cyclocybe aegerita
Primary publication:
Structural insights into the recognition mechanism between an antitumor galectin AAL and the Thomsen-Friedenreich antigen.
Feng L, Sun H, Zhang Y, Li DF, Wang DC
FASEB J 24 3861-8 (2010)
PMID: 20530247

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180509 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Anti-tumor lectin Chains: A, B
Molecule details ›
Chains: A, B
Length: 159 amino acids
Theoretical weight: 17.11 KDa
Source organism: Cyclocybe aegerita
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6WY08 (Residues: 1-158; Coverage: 100%)
Sequence domains: Galactoside-binding lectin
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL, NGA, SIA
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P21
Unit cell:
a: 50.861Å b: 66.711Å c: 56.211Å
α: 90° β: 93.18° γ: 90°
R-values:
R R work R free
0.222 0.209 0.248
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Fungal lectins: structure, function and potential applications.
Varrot et al. (2013)
1 more

3 mentions without citation

Mushroom lectins: specificity, structure and bioactivity relevant to human disease.
Hassan et al. (2015)
2 more