› 3m4d

X-ray diffraction
1.9Å resolution

Crystal structure of the M113N mutant of alpha-hemolysin

Released:
DOI: 10.2210/pdb3m4d/pdb
PDB entry 3m4d coloured by chain, front view.
PDB entry 3m4d coloured by chain, side view.
PDB entry 3m4d coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Molecular bases of cyclodextrin adapter interactions with engineered protein nanopores.
Banerjee A, Mikhailova E, Cheley S, Gu LQ, Montoya M, Nagaoka Y, Gouaux E, Bayley H
Proc Natl Acad Sci U S A 107 8165-70 (2010)
PMID: 20400691

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140713 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-hemolysin Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 293 amino acids
Theoretical weight: 33.27 KDa
Source organism: Staphylococcus aureus
Expression system: Staphylococcus aureus
UniProt:
  • Canonical: P09616 (Residues: 27-319; Coverage: 100%)
Gene names: hla, hly
Sequence domains: Leukocidin/Hemolysin toxin family
Structure domains: Leukocidin/porin MspA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 151.15Å b: 134.59Å c: 132.9Å
α: 90° β: 90.78° γ: 90°
R-values:
R R work R free
0.24 0.24 0.265
Expression system: Staphylococcus aureus

Quick links

Citations

13 review citations

Residue-specific incorporation of non-canonical amino acids into proteins: recent developments and applications.
Johnson et al. (2010)
12 more

5 mentions without citation

The structural biology of β-barrel membrane proteins: a summary of recent reports.
Fairman et al. (2011)
4 more