› 3m4g

X-ray diffraction
2.05Å resolution

H57A HFQ from Pseudomonas Aeruginosa

Released:
DOI: 10.2210/pdb3m4g/pdb
PDB entry 3m4g coloured by chain, front view.
PDB entry 3m4g coloured by chain, side view.
PDB entry 3m4g coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
The structures of mutant forms of Hfq from Pseudomonas aeruginosa reveal the importance of the conserved His57 for the protein hexamer organization.
Moskaleva O, Melnik B, Gabdulkhakov A, Garber M, Nikonov S, Stolboushkina E, Nikulin A
Acta Crystallogr Sect F Struct Biol Cryst Commun 66 760-4 (2010)
PMID: 20606268

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190968 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-binding protein Hfq Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 82 amino acids
Theoretical weight: 9.05 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HUM0 (Residues: 1-82; Coverage: 100%)
Gene names: PA4944, hfq
Sequence domains: Hfq protein
Structure domains: SH3 type barrels.

Ligands and Environments

1 bound ligand:
Ligand ZN 14 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P1
Unit cell:
a: 66.46Å b: 66.587Å c: 68.713Å
α: 91.78° β: 115.32° γ: 119.92°
R-values:
R R work R free
0.203 0.199 0.262
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The bacterial protein Hfq: much more than a mere RNA-binding factor.
Sobrero et al. (2012)
2 more

3 mentions without citation

Structural insights into the dynamics and function of the C-terminus of the E. coli RNA chaperone Hfq.
Beich-Frandsen et al. (2011)
2 more