› 3m6u

X-ray diffraction
1.4Å resolution

Multi-site-specific 16S rRNA methyltransferase RsmF from Thermus thermophilus in space group 43

Released:
DOI: 10.2210/pdb3m6u/pdb
Model geometry
Fit model/data
PDB entry 3m6u coloured by chain, front view.
PDB entry 3m6u coloured by chain, side view.
PDB entry 3m6u coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Multi-site-specific 16S rRNA methyltransferase RsmF from Thermus thermophilus.
Demirci H, Larsen LH, Hansen T, Rasmussen A, Cadambi A, Gregory ST, Kirpekar F, Jogl G
RNA 16 1584-96 (2010)
PMID: 20558545

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + cytosine(1407) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(1407) in 16S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177960 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA small subunit methyltransferase F Chains: A, B
Molecule details ›
Chains: A, B
Length: 464 amino acids
Theoretical weight: 50.88 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SII2 (Residues: 1-456; Coverage: 100%)
Gene names: TTHA1387, rsmF
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P43
Unit cell:
a: 70.979Å b: 70.979Å c: 186.732Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.189
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The mammalian mitochondrial epitranscriptome.
Rebelo-Guiomar et al. (2019)
3 more

1 mention without citation

Multi-site-specific 16S rRNA methyltransferase RsmF from Thermus thermophilus.
Demirci et al. (2010)