Function and Biology Details
Reaction catalysed:
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Sequence domains:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155550 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Growth factor receptor-bound protein 10
Molecule details ›
Chain: A
Length: 108 amino acids
Theoretical weight: 12.64 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
Sequence domains: SH2 domain
Structure domains: SH2 domain
Length: 108 amino acids
Theoretical weight: 12.64 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
- Canonical:
Q60760 (Residues: 514-621; Coverage: 17%)
Sequence domains: SH2 domain
Structure domains: SH2 domain
E3 ubiquitin-protein ligase NEDD4
Molecule details ›
Chain: B
Length: 176 amino acids
Theoretical weight: 20.34 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
Sequence domains: C2 domain
Structure domains: C2 domain
Length: 176 amino acids
Theoretical weight: 20.34 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
- Canonical: P46935 (Residues: 71-246; Coverage: 20%)
Sequence domains: C2 domain
Structure domains: C2 domain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
CHESS BEAMLINE A1
Spacegroup:
P212121
Expression system: Escherichia coli
