› 3m8d

X-ray diffraction
2.44Å resolution

Crystal structure of spin-labeled BtuB V10R1 with bound calcium and cyanocobalamin

Released:
DOI: 10.2210/pdb3m8d/pdb
PDB entry 3m8d coloured by chain, front view.
PDB entry 3m8d coloured by chain, side view.
PDB entry 3m8d coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Conformational exchange in a membrane transport protein is altered in protein crystals.
Freed DM, Horanyi PS, Wiener MC, Cafiso DS
Biophys J 99 1604-10 (2010)
PMID: 20816073

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138877 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vitamin B12 transporter BtuB Chain: A
Molecule details ›
Chain: A
Length: 594 amino acids
Theoretical weight: 66.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P06129 (Residues: 21-614; Coverage: 100%)
Gene names: JW3938, b3966, bfe, btuB, cer, dcrC
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CNC 1 x CNC
Ligand C8E 6 x C8E
Ligand MTN 1 x MTN
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P3121
Unit cell:
a: 82.051Å b: 82.051Å c: 224.457Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.279 0.226 0.275
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Toward the fourth dimension of membrane protein structure: insight into dynamics from spin-labeling EPR spectroscopy.
McHaourab et al. (2011)
2 more

6 mentions without citation

The structural biology of β-barrel membrane proteins: a summary of recent reports.
Fairman et al. (2011)
5 more