› 3ma9

X-ray diffraction
2.05Å resolution

Crystal structure of gp41 derived protein complexed with fab 8066

Released:
DOI: 10.2210/pdb3ma9/pdb
PDB entry 3ma9 coloured by chain, front view.
PDB entry 3ma9 coloured by chain, side view.
PDB entry 3ma9 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of HIV-1 neutralization by affinity matured Fabs directed against the internal trimeric coiled-coil of gp41.
Gustchina E, Li M, Louis JM, Anderson DE, Lloyd J, Frisch C, Bewley CA, Gustchina A, Wlodawer A, Clore GM
PLoS Pathog 6 e1001182 (2010)
PMID: 21085615

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207749 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Transmembrane glycoprotein Chain: A
Molecule details ›
Chain: A
Length: 217 amino acids
Theoretical weight: 24.48 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli
UniProt:
  • Canonical: D0VWW0 (Residues: 1-217; Coverage: 96%)
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1
Fab8066 FAB ANTIBODY FRAGMENT, Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 245 amino acids
Theoretical weight: 26.31 KDa
Source organism: Homo sapiens
Expression system: Not provided
Structure domains: Immunoglobulins
Fab8066 FAB ANTIBODY FRAGMENT, Light Chain Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 22.64 KDa
Source organism: Homo sapiens
Expression system: Not provided
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 41.527Å b: 124.698Å c: 133.657Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.193 0.245
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

Rational design of vaccines to elicit broadly neutralizing antibodies to HIV-1.
Kwong et al. (2011)
5 more

12 mentions without citation

HIV-1 envelope glycoprotein structure.
Merk et al. (2013)
11 more